Figure 6.

PINTA expressed in retinal pigment cells. A, Developmental Western blot probed with anti-PINTA antibodies. Samples were prepared from the indicated developmental stages. Molecular weight markers (in kilodaltons) are indicated to the left. B, Relative PINTA protein levels in various genotypes: sine oculis (flies missing eyes), glass (missing photoreceptor cells), PC-GAL4 (a pigment cell GAL4:P[Gaw-B]LL54), UAS-rpr, and hippo [produces a surplus of pigment cells (Udan et al., 2003; Wu et al., 2003)]. Error bars represent SEM. wt, Wild type. C, D, In situ hybridizations to 8 μm cryostat sections of adult heads hybridized with digoxigenin-labeled RNA probes. C, Antisense pinta RNA probe. D, Sense pinta RNA probe. Br, Brain; L, lamina; M, medulla; R, retina.