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. 2005 Jul 27;25(30):7062–7070. doi: 10.1523/JNEUROSCI.0447-05.2005

Figure 6.

Figure 6.

Association of VILIP-2 with CaV2.1 channels via the IQ-like domain and CBD. Transfected cells were cross-linked in situ with DSP, as described in Materials and Methods. A, Lysates from cells transfected with CaV2.1 plus VILIP-2 myc or CaV2.1ΔCBD plus VILIP-2 myc were subjected to immunoprecipitation by anti-CaV2.1 antibodies or control IgG as indicated in the presence of 10 mm EGTA. B, Lysates from cells transfected with CaV2.1, CaV2.1/1965ST, CaV2.1/IM-AA, and CaV2.1ΔCBD/IM-AA plus VILIP-2 myc were subjected to immunoprecipitation by anti-CaV2.1 antibodies or control IgG in the presence of 10 mm EGTA. Blots were probed with either anti-CaV2.1 (top) or anti-myc (bottom). WT, Wild type.