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. 2005 Jul 27;25(30):7090–7099. doi: 10.1523/JNEUROSCI.2083-05.2005

Figure 5.

Figure 5.

NGF/TrkA signaling is diminished in necdin-null DRG explants. A, B, Neurite outgrowth assay. DRG explants were prepared from E13.5 embryos, treated with NGF for 16 h, and analyzed by phase-contrast microscopy. A, Typical images of NGF-treated DRG explants. B, The neurite length of the explants was measured after NGF treatment for 16 h (explants examined: +m/+p, n = 105; +m/-p, n = 102). Scale bar: A, 200 μm. C, D, Western blot analysis of phosphorylated forms of TrkA and MAPK. DRG explants from E13.5 littermates were cultured in the presence of NGF (20 ng/ml) for the indicated durations. Phosphorylated proteins (pTrkA, pMAPK) were analyzed by Western blotting using phosphoprotein-specific antibodies. The values relative to those of the wild-type controls (=1) at 15 min were presented (mean ± SEM; n = 4). B, D, Graphs show mean ± SEM. *p < 0.01; **p < 0.005; ***p < 0.001.