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. 2005 May 18;25(20):5066–5078. doi: 10.1523/JNEUROSCI.1427-05.2005

Figure 2.

Figure 2.

Estradiol attenuates L-type calcium channel-mediated CREB phosphorylation. A, Estradiol alone resulted in an increase (F = 16.97; **p < 0.01) in CREB phosphorylation (left 2 bars). However, estradiol treatment in the presence of a 20 mm K+ stimulus led to a significant (**p < 0.01) attenuation of depolarization-induced CREB phosphorylation (middle 2 bars). Pretreatment with the L-type calcium channel blocker nifedipine (NIF; 5 μm) eliminated depolarization-dependent CREB phosphorylation and unmasked estradiol-induced CREB phosphorylation (right 2 bars; **p < 0.01). B, Estradiol produced a rightward shift in the cumulative percentile of CREB fluorescence under basal conditions. C, Conversely, estradiol generated a leftward population shift in CREB phosphorylation after a 20 mm K+ depolarization. Error bars indicate SEM.