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. 2005 May 18;25(20):5066–5078. doi: 10.1523/JNEUROSCI.1427-05.2005

Figure 4.

Figure 4.

The effects of estradiol occur via action at the plasma membrane, are mimicked by the mixed estrogen receptor agonist/antagonist 4-hydroxy-tamoxifen, and are blocked by the estrogen receptor antagonist ICI 182,780. A, B, The membrane-impermeable estrogen analog EBSA mimicked the bidirectional effects of estradiol on CREB phosphorylation [F = 42.02 and 79.60; **p < 0.01 vs basal (A) and 20 mm K+ (B)]. C, D, The effect of 4-hydroxy-tamoxifen (4-OH-TMX) also paralleled the effect of estradiol [F = 31.15 and 41.27; **p < 0.01 vs basal (C) and 20 mm K+ (D)]. E, ICI 182,780 (ICI; 100 nm) eliminated both the estradiol-induced increase in CREB phosphorylation (F = 81.28; **p < 0.01 vs 17βE) and the estradiol-induced attenuation of L-type calcium channel-mediated CREB phosphorylation (**p < 0.01 vs 20 mm K+ plus 17βE). Error bars indicate SEM.