Figure 4.

The effect of acute treatment (A) with fluoxetine, reboxetine, and desipramine on the release of glutamate from rat hippocampal synaptosomes. The three drugs were administered intraperitoneally (10 mg/kg), and the rats were killed after 3 h. Synaptosomes were superfused and stimulated as in previous figures; the stimulus-evoked overflow is reported. Data represent the means ± SEM of three to four separate experiments run in triplicate. No significant differences were found (two-tailed Student's t test). Expression levels of total αCaM kinase II (B) and αCaM kinase II phosphorylated on Thr²⁸⁶ (C) in synaptosomes from rats acutely treated with antidepressants are shown. Expression levels were normalized for level of β-actin in the same blotted membrane. Representative immunoreactive bands are shown. Data represent the means ± SEM (percentage treated vs control) of three separate experiments run in triplicate; n.a., not assessed; iono, ionomycin. No significant differences were found (two-tailed Student's t test).