Table 1.
Effect of chronic treatment with desmethylimipramine (DMI) on the release of glutamate and GABA from rat hippocampal synaptosomes
|
|
25 mm KCl |
1 μm lonomycin |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
|
|
Control |
|
DMI |
Control |
|
DMI |
||||
| Glutamate | 4.03 ± 0.270 (4) | 2.66 ± 0.309 (4) | 5.62 ± 0.803 (6) | 4.31 ± 0.721 (6) | ||||||
| p <0.05 | NS | |||||||||
| GABA | 1.15 ± 0.083 (6) | 1.21 ± 0.089 (6) | 0.677 ± 0.128 (5) | 0.675 ± 0.120 (5) | ||||||
|
|
|
NS |
|
|
NS |
|
||||
DMI was administered by means of osmotic minipumps (10 mg/kg daily). After 14 d of treatment, animals were killed for release experiments. Synaptosomes were exposed in superfusion to a 90 s pulse of 25 mm KCl or 1 μm ionomycin at t = 39 min, and the stimulus-evoked overflow of endogenous glutamate and GABA was monitored. The stimulus-evoked overflow was calculated by subtracting the neurotransmitter content in the basal release from the release evoked by stimulation (see the legend to Fig. 1). Data represent the means ± SEM of the number of experiments indicated in parentheses. Values are expressed in nanomoles per milligram of protein. NS, Nonsignificant.