Figure 5.

Histology of the sciatic nerves from vehicle- or PKI166-treated rats after nerve injury. Myelin sheaths are visualized by toluidine blue staining on semithin sections of distal sciatic nerves from rats treated with either DMSO or PKI166. Two days after nerve transection, disorganization of myelin sheaths is seen in the DMSO-treated animal (Cut-DMSO), including separation of compact myelin and unwinding of myelin layers (arrowheads). In the PKI166-treated animal (Cut-PKI166), layers of myelin are still compact and smooth with no visible sign of demyelination in most of the Schwann cells. In some cases, compact myelin sheaths are actually retained even in the absence of the axons (arrows).