Figure 6.

Neuregulin pulse-chase and Schwann cell demyelination in culture. Neuron/Schwann cell cocultures that had myelinated for 4 weeks were treated with neuregulin (30 ng/ml) for various times (30 min, 1, 3, 6, and 48 h), and the culture medium was removed. Cultures were washed with PBS and then incubated continually in fresh medium for the remainder of the experimental period (48 h). Cultures were fixed and immunostained for MBP and scored for demyelination. The mean values and SEM presented for each data point are from at least 80 random fields pooled from a representative experiment. Similar results were observed in triplicate independent experiments.