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. 2005 Apr 13;25(15):3741–3751. doi: 10.1523/JNEUROSCI.0152-05.2005

Figure 6.

Figure 6.

Stress-induced activation of JNK increases APP phosphorylation in the cell body but not in the processes. a, Exogenous expression of JIP-3 or treatment with sorbitol increases APP phosphorylation. Immunoblots of lysates from JIP-3-FLAG-transfected or sorbitol-treated CAD cells were probed with antibodies to pAPP. Control cells were treated with DMSO. The Bcr blot shows equal protein load. b-k, Immunolocalization of pAPP (b, e, h, k), total APP (tAPP; d, g), and FLAG-tagged JIP-3 (j) in CAD cells treated with anisomycin (b, c), DMSO (d-f), or sorbitol (g-i) or transfected with JIP-3-FLAG and then treated with anisomycin (j, k). Note that treatment with either anisomycin or sorbitol causes accumulation of pAPP in the cell body but not at neurite terminals (compare b and h with e), whereas expression of JIP-3 leads to increased accumulation of pAPP both in the cell body and at process terminals (j, k). Also note that drug treatment does not increase the level of total APP in cells (compare g with d). Arrows point to neurite terminals. c, f, i, Phase-contrast micrographs. Scale bars: (in c) b-i, 30 μm; (in k) j, k, 50 μm.