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Inhibition of protein phosphatases PP1 and PP2A before stimulation with DHPG enhances mGluR5-mediated ERK2 phosphorylation. Okadaic acid (A) or calyculin A (CalA) (B) pretreatment enhances ERK2 phosphorylation by DHPG (D) (10 μm; n = 5). Asterisks indicate a significant increase over control; # indicates a significant difference from DHPG treatment alone.
