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. 2005 Apr 20;25(16):4040–4051. doi: 10.1523/JNEUROSCI.4115-04.2005

Figure 7.

Figure 7.

NR2B-NMDARs act at silent but not at functional synapses in TTX-treated neurons. A, Representative mEPSCs recorded at -60 mV holding potential from control (Ctrl, left traces) and TTX-treated (TTX, right traces) neurons. The second and third traces were obtained in the presence of 1 μm ifenprodil and 100 μm APV, respectively. Bottom, Representative traces for BotxA-treated (BotxA) neurons, showing the absence of miniature synaptic events. B, Plot of decay time versus amplitude of mature mEPSCs. Ifenprodil (4 μm) and APV (100 μm) sensitivities of mEPSCs are indicated in the second and third rows, respectively. Cumulative plot of decay time of mEPSCs is indicated in the bottom row, showing that NR2B-NMDARs do not make a major contribution to mEPSCs. C, Fluctuations of membrane currents from control (Ctrl), TTX-treated (TTX), and TTX plus BotxA-treated (TTX + BotxA) neurons. Note that the fluctuation of membrane currents in TTX-treated neurons is significantly larger than in Ctrl (**p < 0.01) and TTX + BotxA (*p < 0.05) neurons.