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Secreted sortilin reduces degradation of proBDNF. Conditioned media from 293 cells infected with adenovirus (Ad) expressing GFP or soluble sortilin were mixed (4 h at 4°C) with conditioned media from cells expressing proBDNF-His. These mixtures were then incubated with plasmin at the indicated concentration for 1 h, followed by SDS-PAGE and Western blot analysis using anti-sortilin (top panels) or anti-BDNF (bottom panels) antisera.
