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. 2005 May 4;25(18):4659–4671. doi: 10.1523/JNEUROSCI.0565-05.2005

Figure 4.

Figure 4.

Synaptophysin immunofluorescence and dendrite spine density are decreased in NCAM-EC cingulate cortex. A, Immunofluorescent staining for synaptophysin was analyzed in cingulate cortex (layers II/III) of adult WT and NCAM-EC transgenic mice. Fluorescent puncta rings surrounding nonlabeled soma are shown in confocal images by arrows. Control staining with nonimmune IgG is shown in the inset. B, Spines on apical dendritic branches in the cingulate cortex (layers II/III) of adult WT (Thy1/YFP line H) and transgenic mice (NCAM-EC/Thy1) were calculated. Arrows indicate some of the spines on the dendrite. *p < 0.05, from the WT control. Scale bar, 10 μm.