NeuN-positive neuron loss in the SNC of Wnt-BDNFKO mice was not detected. A, NeuN-positive cells were counted in the SNC, defined by outlining the TH-stained population of neurons on adjacent sections, and estimates were obtained for total numbers of neurons in the SNC at P21 (n = 4 for Wnt-BDNFKO and heterozygous; n = 3 for wild type; p = 0.5093). B, Stereological estimates of calbindin-positive cell population in the SNC. C, Estimates of calretinin-positive cell population in the SNC. For both experiments, n = 3/genotype. No significant difference was found between genotypes by one-way ANOVA. White bars, Wnt-BDNFKO; gray bars, heterozygous; black bars, wild type.