Figure 3.

A, Deblurred fluorescence image of a growth cone and process of a neuron expressing tPA-EGFP (green) that was stained with anti-tubulin antibodies (blue) and Texas Red phalloidin (red). The arrowheads show DCGs that colocalize with microtubules, whereas the arrows show DCGs that colocalize with actin filaments. B, Deblurred fluorescence image of the soma of an untransfected neuron that was stained with anti-tubulin antibodies (blue) and FITC phalloidin (green). C, Deblurred fluorescence image of processes of a hippocampal neuron stained with Texas Red phalloidin showing that actin filaments are significantly disrupted by incubation with 10 μm cytochalasin D for ∼1 h. The inset shows stained, undisrupted actin filaments in part of a process on the same display scale. D, Deblurred fluorescence image of part of the soma and proximal processes of a hippocampal neuron stained with anti-tubulin antibodies showing that microtubules are significantly disrupted by incubation with 30 μm nocodazole for ∼1 h. The inset shows stained, undisrupted microtubules in the soma and proximal processes on the same display scale. Scale bars: A, C, 5 μm; B, D, 10 μm; C (inset), 5 μm; D (inset), 10 μm.