Figure 2.

Discharge of an Nb-labeled cholinergic neuron. A-D, Record of Nb+/ChAT+ unit discharge (Fig. 1 A, #c30u13) together with EEG and EMG activity during 10 s periods of aW (A), SWS (B), tPS (C), and PS (D). The unit fired during aW, virtually ceased firing during SWS, resumed firing during tPS, and discharged maximally during PS. As evident in the expanded 0.5 s traces (bottom), the unit discharged in bursts of spikes with theta EEG activity that was present intermittently during aW, briefly at the end of tPS, and continuously during PS. Calibration: 1 s, 1 mV (EEG, EMG), 1.5 mV (Unit).