MCH1R is expressed in both Dyn and Enk neurons within the AcSh. a, Fluorescent microscopy shows the expression pattern of MCH1R, Enk mRNA, and Dyn mRNA in the AcSh. Confocal microscopy shows the colocalization of MCH1R with Enk (b) or Dyn (c). Horizontal arrows indicate examples of colocalization; vertical arrows indicate examples of cells that express MCH1R but not the neuropeptide. AC, Anterior commissure; Cy, cyanine.