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Role of IGF-1 in low-potassium-induced sustained ERK activation. IGF-1 induces a rapid and transient rise in ERK but prevents sustained ERK in low potassium. After the potassium change (low K+), CGN extracts were isolated from cultures treated with U0126 (10 μm), IGF-1 (100 ng/ml), and IGF-1 plus U0126. Western blotting was done to detect activated ERK (pERK) and total ERK (ERK). Error bars indicate SE.
