Figure 3.

Adenoviral-mediated CA MEK (Ad-CA MEK) overexpression attenuates IGF-1-inhibited PM damage. A, Fluorescence microscopy of CGNs infected with Ad-GFP, Ad-CA MEK infected CGNs. An antibody against HA epitope was used to detect CA MEK. B, Quantification of the percentage of total PI-positive cells in Ad-GFP- and in Ad-CA MEK-infected cultures. ^**p < 0.01 compared with Ad-GFP+PI alone. ⁰⁰⁰p < 0.001 compared with Ad-GFP+PI in IGF-1-treated cultures. Error bars indicate SE. C, Western blot analysis for pERK in Ad-GFP- and Ad-CA MEK-infected cultures.