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. 2005 Mar 16;25(11):2895–2905. doi: 10.1523/JNEUROSCI.5217-04.2005

Figure 5.

Figure 5.

Synaptic scaling does not affect vesicle recycling dramatically. A1, Distal dendrites of pyramidal neuron, labeled with Alexa Fluor 594. A2, Presynaptic terminals were labeled with electrical stimulation (30 s at 20 Hz) in the presence of 15 μm FM1-43. A3, After destaining (1500 stimuli at 10 Hz), FM1-43 puncta have disappeared. Only FM1-43 puncta that touched the dendrites of the filled neuron were included in the analysis. Scale bars, 5 μm. All images were taken from the same field of view. B, Cumulative distribution of intensity values of FM1-43 puncta labeled by weak electrical stimulation (20 stimuli at 0.5 Hz). Data are from 111 control and 113 puncta; there are nine neurons in each condition. Intensity values are shown in arbitrary units (a.u.). C, Cumulative distribution of intensity values of FM1-43 puncta labeled by high K+ protocol. Data are from 1404 control and 1191 TTX puncta; there are 14 neurons in each condition. D, Destaining time course for individual puncta in control neurons. Thick black dots are average values. E, Average destaining time course for control (black) and TTX puncta (gray). Error bars represent SD for 160 control and 103 TTX puncta (6 neurons in each condition). Puncta were destained maximally with 1500 stimuli at 10 Hz, because additional stimulation did not result in more destaining.