Figure 2.

B7-H1 protein expression in cultured murine microglial cells, astrocytes, and splenocytes. The expression of B7-H1 and MHC class II (HLA-DR) was assessed by flow cytometry in purified and cultured microglial cells, astrocytes, and splenocytes maintained in the absence or presence of IFN-γ (500 U/ml; 48 h). Histograms show staining with the designated antibodies (open) underlaid with the respective isotype controls (filled). The expression analysis was performed with glial cell cultures from five different animal preparations. A representative experiment is shown.