Figure 3.

mRNA expression of B7 family members in cultured murine microglia and astroglia. Microglia and astrocytes cultured in the absence or presence of IFN-γ (500 U/ml) were harvested after 48 h of induction and analyzed for the expression of the indicated mRNA by QRT-PCR. A, B7.1 (CD80); B, B7.2 (CD86); C, B7-H1 (PD-L1); D, PD-L2. Splenocytes and PHA-stimulated splenocytes were used as controls. Bars and numbers represent the relative gene expression of indicated molecules calculated in relation to unstimulated splenocytes (set to 1). Data represent expression analysis from glial cultures from three different animal preparations (mean ± SEM).