Figure 7.

Upregulation of B7-H1 expression by CNS-infiltrating macrophages and CNS resident microglia of mice with EAE. A, EAE was induced by subcutaneous immunization with 50 nmol of PLP139-151 in CFA and intravenous injection of 300 ng of pertussis toxin, and disease score was determined as described in Materials and Methods. Clinical disease symptom speaked at day 14 and subsequently declined until day 20 after disease induction. B, CNS cells were isolated from diseased animals at the peak of clinical disease (day 14) and during remission (day 20). B7-H1 expression by CD45^high/CD11b⁺ macrophages (R1) and CD45^low/CD11b⁺ (R2) microglia was determined by flow cytometry. Data are representative of six individual mice. Error bars indicate SEM. B7-H1 expression is higher during remission (t test; ^*p < 0.02; ^**p < 0.01).