Figure 6.

Localization of the SHPS-1 mutants ΔV, ΔC1#1, and ΔC1#2 in cultured rat hippocampal neurons. A-D, Neurons were transfected with expression vectors for wild-type (WT) mouse SHPS-1 (mSHPS-1) (A) or for the SHPS-1 mutants ΔV(B), ΔC1#1 (C), or ΔC1#2 (D) at 12 DIV. They were fixed at 14 DIV and immunostained with P84 (A, B, D) or FG2 (C) mAbs to SHPS-1. Schematic structures of the wild-type and mutant proteins are shown at the side of each image. Scale bar, 50 μm. Results are representative of three separate experiments. E, Quantitation of the axon/dendrite fluorescence ratio for exogenously expressed GFP, wild-type mSHPS-1, or the SHPS-1 mutants ΔV, ΔC1#1, or ΔC1#2. Data are means ± SE of values from 8 to 16 different cells. ^*p < 0.05 for the indicated comparisons (Student's t test).