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. 2005 Jan 5;25(1):260–270. doi: 10.1523/JNEUROSCI.3165-04.2005

Figure 2.

Figure 2.

Reconstitution of glutamatergic transmission on HEK293 cells. The recordings obtained from the cocultures of neurons and HEK293 cells expressing GluR2 alone or with adhesion molecules SynCAM-NL1. A, B, Examples of whole-cell recordings from SynCAM- or NL1-expressing HEK293 cells exhibit absence of spontaneous electrical activity (left). Spontaneous activity was present in both SynCAM and NL1 coexpression with GluR2 (middle) and completely inhibited by CNQX, indicating that this activity was glutamatergic (right). C, Current-clamp recordings from neurons in cocultures displayed spontaneously firing neurons as a result of spontaneous network activity (1.3 ± 0.2 Hz; n = 6). D, Large synaptic activities observed in the neurons under whole-cell voltage clamp with electrodes filled with QX-314 to eliminate action potential firing (n = 6). E, Average amplitude was significantly larger in neurons than in the HEK293 cells (neurons, n = 6; SynCAM, n = 23; NL1, n = 17; p < 0.01). F, Spontaneous event frequency (Event Freq.) in neurons was in the same order as that of the HEK293 activity (neurons, n = 6; SynCAM, n = 23; NL1, n = 17; p > 0.05).