Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2005 Jan 5;25(1):184–191. doi: 10.1523/JNEUROSCI.4269-04.2005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2005 Society for Neuroscience 0270-6474/05/25184-08.00/0

PMC Copyright notice

Figure 3. — Free-radical production in TH neurons of the SNc in UCP2 WT/KO mice. A, B, Representative photomicrographs of in situ ROS production assessed by the oxidation of DHE to ethidium by . Left photomicrographs show a representation of double exposure (A) for ethidium (red) and Hoechst nuclear staining (blue) or triple exposure (B) for ethidium, Hoechst nuclear staining, and TH immunofluorescence (green) in UCP2 WT mice. C, D, In UCP2 KO mice (right photomicrographs), representative findings for ethidium and Hoechst nuclear staining (C) with TH immunofluorescence (D). Scale bar: (in C) A-D, 10 μm. E, Quantification of the number of red fluorescent mitochondria within TH cells revealed a significant increase in UCP2 KO mice compared with their WT controls (n = 6, n = 5, respectively). ^*Significant with respect to WT controls (p < 0.05). Error bars represent SEM.

Inline graphic — Free-radical production in TH neurons of the SNc in UCP2 WT/KO mice. A, B, Representative photomicrographs of in situ ROS production assessed by the oxidation of DHE to ethidium by . Left photomicrographs show a representation of double exposure (A) for ethidium (red) and Hoechst nuclear staining (blue) or triple exposure (B) for ethidium, Hoechst nuclear staining, and TH immunofluorescence (green) in UCP2 WT mice. C, D, In UCP2 KO mice (right photomicrographs), representative findings for ethidium and Hoechst nuclear staining (C) with TH immunofluorescence (D). Scale bar: (in C) A-D, 10 μm. E, Quantification of the number of red fluorescent mitochondria within TH cells revealed a significant increase in UCP2 KO mice compared with their WT controls (n = 6, n = 5, respectively). ^*Significant with respect to WT controls (p < 0.05). Error bars represent SEM.