Figure 11.

Effects of β-PMA and PD169316 on SERT internalization. HEK-293 cells transiently transfected with hSERT were surface biotinylated using cleavable biotin, incubated with vehicle, β-PMA (1.0 μm), or PD169316 (10 μm) for 30 min at 37°C, washed, and stripped with cell-impermeant MesNa. Biotinylated SERTs were isolated with avidin, and internalized SERT was quantified after Western blot analysis (as described in Materials and Methods). A, Representative Western blots for total surface SERT before and after MesNa treatment (4°C) and for the internalized SERT (37°C). B, Quantification of internalized SERT. Data are expressed as a fraction of the total surface SERT internalized, and averaged data from three separate experiments are shown as the mean ± SEM. Values significantly different from vehicle-treated cells (^*p < 0.01; one-way ANOVA with Bonferroni post hoc analysis).