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. 2005 Jan 5;25(1):29–41. doi: 10.1523/JNEUROSCI.3754-04.2005

Figure 5.

Figure 5.

Effect of PD169316 and β-PMA on SERT-PP2Ac or SERT-syntaxin 1A interaction. Synaptosomes were treated with 10 μm PD169316 or 1 μm β-PMA and/or vehicle for 30 min. The synaptosomes were solubilized and immunoprecipitated with the SERT-specific antibody SR-12. Immunoprecipitates were subjected to SDS-PAGE and immunoblot analysis for PP2Ac or syntaxin 1A (as described in Materials and Methods). Parallel experiments using muscle extract and preimmune IgG were performed to validate the specificity of SERT-specific antibody SR-12 and SERT interactions. Note that no PP2Ac or syntaxin 1A is seen in muscle extract using SR-12 antibody or in midbrain extract using preimmune IgG. A, Representative immunoblots are shown for SERT-PP2Ac and SERT-syntaxin 1A associations from synaptosomes treated with 10 μm PD169316 or 1 μm β-PMA and or vehicle. B, The bar plot displays averaged protein band densities from three separate experiments that are presented ± SEM. *p < 0.01; significant differences compared with vehicle-treated controls (one-way ANOVA with Tukey-Kramer post hoc analysis).