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. 2005 Aug 3;25(31):7121–7133. doi: 10.1523/JNEUROSCI.5221-04.2005

Figure 6.

Figure 6.

VGLUT1 and VGLUT2 are coexpressed in subsets of neocortical neurons in situ. High-power micrograph of emulsion-dipped autoradiograms illustrating the coexpression of VGLUT2 mRNA transcripts (detected with radiolabeled probe) and the VGLUT1 mRNA (detected with a digoxigenin-labeled probe) in P10 (left) and adult (right) cortical regions. A, B, Parietal cortex layer II/III. C, D, Auditory cortex layer II/III. Note that most neurons in layer II/III coexpress VGLUT1 and VGLUT2 at P10, whereas labeling is only slightly above background in few adult cells (arrows). E, F, Parietal cortex layer IV. G, H, Auditory cortex layer V/VI. Note the high level of VGLUT2 expression and coexpression with VGLUT1 at P10 and subpopulation of neurons expressing moderate levels in adult (arrows). I, J, Posterior cingulate cortex (retrosplenial cortex). Note moderate levels of VGLUT2 expression and coexpression with VGLUT1 at P10 and in adult (arrows). Scale bar, 50 μm.