Figure 1.

Communication of the 5-HT₇ receptor with different G-proteins. A, Membranes were prepared from Sf.9 cells expressing or not expressing the 5-HT₇ receptor together with recombinant G-proteins as indicated and then incubated with [³⁵S]GTPγS in the presence of either vehicle (H₂O) or 1 μm 5-HT. Immunoprecipitations were performed with appropriate antibodies directed against indicated Gα subunits. Data points represent the means ± SEM from at least four independent experiments performed in duplicate. Statistically significant differences between values obtained with or without stimulation are indicated (^*p < 0.01). B, Expression analysis of the 5-HT₇ receptor and G-proteins. Sf.9 cells infected with baculoviruses encoding for recombinant, myc-tagged 5-HT₇ receptor and for different heterotrimeric G-proteins (Gs, Gi₂, G12, G13, and Gq) or with a baculovirus alone (Bac) were subjected to Western blot analysis with anti-myc or appropriate anti-Gα subunit antibodies. The molecular weight marker is indicated to the left.