Figure 6.

Effects of extracellular UTP on α,β-meATP-induced currents in rat cultured DRG neurons. The whole-cell variant of the patch-clamp method was used to record membrane currents. Cells were held at -60 mV. α,β-meATP and UTP were locally applied by an experimental protocol similar to that used in Figure 1 B. The patch pipettes were filled with a GDP-β-S (300 μm)-containing solution. Aa, Facilitation by UTP (3 μm) of the α,β-meATP (3 μm)-induced current; original tracing. Ab, Mean ± SEM of seven experiments similar to that shown in Aa. Ba, Failure of UTP (3 μm) to facilitate the α,β-meATP (3 μm)-induced current in the presence of the PKC-inhibitor Gö 6976; original tracing. Superfusion with Gö 6976 started 10 min before the first application of α,β-meATP and continued for the duration of the whole experiment. Bb, Mean ± SEM of eight experiments similar to that shown in Ba. ^*p < 0.05, statistically significant difference from the preceding column.