Figure 2.

Intracellular Ca²⁺ mobilization in neurons treated by nerve growth factors and NAP. Ca²⁺ mobilization into the cytoplasm and nucleoplasm of treated cortical neurons monitored by the fluorescence of Ca²⁺-bound rhod-2/AM (top). Cortical neurons were incubated (5 min, 25°C, in serum-depleted MEM) with NGF (100 ng/ml), NAP (10¹² m), NT-3 (100 ng/ml), and BDNF (100 ng/ml) under a confocal microscope. Bottom, The same neurons visualized by phase contrast in transmitted light (n = 3).