Skip to main content
. 2005 Aug 10;25(32):7420–7428. doi: 10.1523/JNEUROSCI.0333-05.2005

Figure 4.

Figure 4.

Single stranded DNA examined for nick formation in neurons treated by nerve growth factors and NAP. Alkaline gel electrophoresis of DNA was used to examine nicks in single-stranded DNA. DNA was extracted from nuclei of rat brain cortical neurons, untreated (lane 1), or incubated (5 min, 25°C) with 100 ng/ml NGF, BDNF, or NT-3 and with 10-12 m NAP, as indicated. As a positive control for nicked DNA, DNA was treated with DNase I. Isolated nuclei of cortical neurons were incubated for 5 min at 37°C with DNase I (10 U; RNase free; D7291; Sigma). Single-stranded DNA in the samples was stained with ethidium bromide (1 μg/ml) and photographed under UV illumination. MW, Molecular weight.