Figure 3.

fmn is a dDAT mutation. a, Summary of the mapping of fmn by meiotic recombination. The map of the dDAT region is illustrated, showing locations of the dominant genetic markers and P-element insertions used for recombinational mapping of fmn. b, Northern blot analysis of dDAT expression. The blot was hybridized with the complete dDAT ORF (left), a 3′ partial ORF (right), or rp49 sequences (positive control). A 3′-deleted, truncated dDAT mRNA is expressed in fmn mutants. See Materials and Methods for details. c, Structures of dDAT genome and cDNA of wild type and fmn. In fmn, exon 6-intron 6 splicing is affected, presumably because of the heterologous DNA insertion into intron 6. This aberrant splicing results in the termination of dDAT protein at residue 343 in fmn. d, e, Rescue of fmn by neuronal dDAT expression, using elav-Gal4 as a driver. Quantitative activity and rest analysis was performed for w and fmn mutants using data collected in DD. The presence or absence of ELAV-GAL4 and the UAS-dDAT responder transgenes are indicated by + or -. The results indicate a partial rescue of the fmn phenotype by transgenic dDAT expression. Differences from nontransgenic and both single transgenic flies (n = 10) are statistically significant using Student's t test (^*p < 0.05).