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Orexin neurons express CCKAR. A, Orexin-ir neurons were located in the lateral hypothalamic area (Alexa 488, green). B, CCKAR-ir was observed in the same area (Alexa 594, red). CCKAR-ir was observed on the somata and dendrites. C, Immunoreactivity for orexin and CCKAR overlaps in the merged image. Arrowheads indicate that orexin neurons express CCKAR. The arrow indicates that a non-orexin neuron in the same area expressed CCKAR as well. Scale bar, 50 μm.
