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Simultaneous recording of calcium imaging and slice patch clamp. A, The neuron subjected to calcium imaging was whole-cell patch clamped. Membrane potential was held at -60 mV. CCK-8S (100 nm) was applied by bath application during the period represented by the bar. The experiment was performed in the presence of TTX (1 μm). B and C summarize an inward current and Δ ratio alteration obtained in A. Values are mean ± SEM. *p < 0.05.
