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. 2005 Jul 20;25(29):6721–6728. doi: 10.1523/JNEUROSCI.0760-05.2005

Figure 1.

Figure 1.

Targeting the Bcl-x gene in TH+ cells. A, A TH-Cre construct possessing a 9.0 kb fragment of the rat TH promoter, synthetic intron, Cre cDNA, and an SV40 polyadenylation sequence. Numbered arrows represent primers used to screen for the Cre-coding sequence. B, A map of the Bcl-x locus containing a floxed version of the gene and the predicted product of Cre-mediated recombination, including the elimination of a region of the 5′-untranslated sequence, exons 1 and 2, and intervening DNA. Numbered arrows represent PCR primers. C, A montage of ethidium bromide-stained 2.0% agarose gel of PCR products amplified from ventral midbrain DNA of indicated mouse strains. PCR using primers III and V demonstrates selective deletion of DNA between loxP sites in the presence of Cre recombinase in vivo. Each lane represents midbrain DNA samples isolated from an individual mouse of the indicated genotype.