Figure 4.

Bcl-x immunoreactivity is lost in a subset of TH-positive neurons of conditional knock-out mice. Tissue sections from the substantia nigra were taken from adult TH-Cre negative, fl/fl Bcl-x (undeleted) (A-F) and TH-Cre positive, fl/fl Bcl-x (Bcl-x-deleted) (G-L) mice, stained for the presence of TH (red) and Bcl-x (green) and visualized by confocal microscopy. Some TH-positive cells in the TH-cre, fl/fl Bcl-x substantia nigra continue to express Bcl-x (I, arrowheads), whereas others clearly do not (L, arrowhead). Magnification: A-C, G-I, 40×; D-F, J-L, 100×.