Figure 5.

SC stimulation monosynaptically excites NG cells. A, SC stimulation evokes EPSCs with a short and constant latency in voltage-clamped NG cells. The left inset shows the differentiated traces illustrating onset latency; the right inset depicts the position of recording and stimulating electrodes. B, Under conditions favoring the recording of NMDA currents (20 μm DNQX; holding potential, +40 mV), monosynaptic EPSCs were also evoked in the same neuron. For each panel, 10 superimposed sweeps are shown. Onset latency distributions for both AMPA-mediated (C₁) and NMDA-mediated (C₂) EPSCs were similar, confirming that the glutamatergic input from the SCs onto this cell was monosynaptic.