Table 2.
Emergence, expansion, and maturation of OPCs from PDGFRα mutant mice
|
|
OPC (04+) |
Oligos (04+, MBP+) |
||||||
|---|---|---|---|---|---|---|---|---|
| Genotype |
Day 7 |
Day 14 |
Day 21 |
Day 14 |
Day 21 |
|||
| +/+ | 80 ± 17 | 1333 ± 330 | 1497 ± 242 | 80 ± 29 | 271 ± 119 | |||
| +/− | 76 ± 10 | 229 ± 53* | 201 ± 64* | 384 ± 36* | 1110 ± 336 | |||
| −/− |
48 ± 13 |
28 ± 5*
|
25 ± 3*
|
35 ± 11 |
16 ± 4 |
|||
Numbers of 04-positive OPCs and MBP+ oligodendrocytes (oligos) in forebrain explants established from E13.5 αR2-4 mice are shown. Explants plated on cover slips (6 wells per pup) were maintained from 1 to 3 weeks in culture, and values represent total cell counts (mean ± SEM) per well from at least three experiments for each genotype and time point. Animals were genotyped as described in Materials and Methods: +/+, wild type; +/−, pdgfra hemizygous; −/−, pdgfra homozygous null. *p < 0.05, differences between wild type were statistically significant (Student's t test).