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. 2005 Apr 6;25(14):3571–3577. doi: 10.1523/JNEUROSCI.4971-04.2005

Figure 2.

Figure 2.

G-protein activation increases the single-channel open probability of nAChRs. A, Direct activation of G-proteins on nAChR single-channel activity recorded from an excised membrane patch in the inside-out configuration (6 s trace). Single-channel currents were evoked in the presence, but not the absence, of ACh (5 μm) in the pipette for control and effects of GTPγS and GDPβS application. B, Bar graph of the effect of G-protein activation on NPo of nAChR channels (n = 8). Holding potential, -60 mV. C, D, Unitary current amplitudes (C) and closed-time distributions (D) from eight inside-out patches exposed to control buffer and 0.2 mm GTPγS. Frequency of events was normalized (Norm. frequency) to the total number of events for each condition. Data points represent mean ± SEM. **p < 0.01.