Skip to main content
. 2005 Apr 6;25(14):3701–3711. doi: 10.1523/JNEUROSCI.4346-04.2005

Figure 4.

Figure 4.

Inhibition of SDM-induced TNF-α release into the BV2 cell-culture media by the MMP-3 inhibitor NNGH. A, SDM-mediated TNF-α release from microglia was abolished by NNGH pretreatment. TNF-α levels were measured in BV2 cell-culture media after 24 h incubation with either SDM or SDM with NNGH. Control, Untreated; SDM, concentrated SDM (50 μg/ml); NNGH+SDM, 30 min pretreatment of NNGH (60 μm) and then SDM (50 μg/ml). B, Enzymatic activity of MMP-3 was essential to induce TNF-α in microglia. TNF-α levels were measured in BV2 cell-culture media after 24 h incubation with pMMP-3, cMMP-3, or cMMP-3 with NNGH. Control, Untreated; pMMP-3, pMMP-3 at 400 ng/ml; cMMP-3, cMMP-3 at 400 ng/ml; NNGH+cMMP-3, 30 min pretreatment of NNGH (60 μm) and then cMMP-3 (400 ng/ml). Values represent the mean ± SD (n = 4).