Figure 7.

The group I metabotropic glutamate receptor agonist DHPG depresses NMDA-EPSC amplitude via activation of mGluR1 but not mGluR5. a, Effect of DHPG (50μm) on NMDA-EPSCs in the presence of the mGluR5 antagonist MPEP (10μm; n = 6; filled squares) and in the presence of the mGluR1 antagonist LY367385 (100-300 μm; n = 6; combined data, open squares). Records show NMDA-EPSCs in two representative neurons, before and during DHPG in the presence of MPEP (top records) or LY367385 (bottom records). Calibration: 20 pA, 20 ms. b, Summary of the effect of okadaic acid (OA) (1 μm, intracellular), BAPTA (20 mm, intracellular), CK1-7 (100 μm), MPEP (10 μm), and LY367385 (100-300 μm) on DHPG-induced inhibition of NMDA-EPSCs. Data are expressed as percentage of baseline (absence of DHPG). ^*p < 0.05, ^***p < 0.001 compared with DHPG alone (unpaired two-tailed t test).