Figure 3.

Relationship between interkinetic nuclear migration, Tis21-GFP expression, and BrdU incorporation of NE/RG cells and basal/SVZ progenitors. White nuclei, GFP- NE cells undergoing proliferative divisions; dark gray nuclei, GFP+ NE/RG cells and basal/SVZ progenitors undergoing neurogenic divisions; hatched boxes, S phase nuclei incorporating BrdU; dark gray polygons, neurons. Lines flanked by arrows indicate the length of T_C - T_S (the total length of the cell cycle minus the S phase) for GFP- versus GFP+ NE/RG cells and of T_{G2 +M} (the G₂ phase plus the minimum period at the end of the S phase necessary to incorporate detectable amounts of BrdU plus the fraction of M phase needed to identify condensed chromatids or detect anti-phosphohistone H3 immunoreactivity) for GFP+ NE/RG cells versus GFP+ basal/SVZ progenitors. The slope of the apical-to-basal migration of nuclei indicates the difference in the length of G₁. For details, see text and supplemental material (available at www.jneurosci.org).