Table 1.
Proportion and cell-cycle parameters of GFP − and GFP+ NE/RG cells
|
|
Proportiona (%) |
Cell-cycle parametersb (hours) |
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Stage |
VZ cells |
t0 h
|
t24 h
|
TC—TS
|
TS
|
TC
|
TG2 + M
|
TG1
|
(% ± sd) GF |
|||||
E10.5 | GFP − | 95 | 90 | 8.5 | 2.4 | 10.9 | <2.0 | >6.5 | 83 ± 3 | |||||
GFP + | 5 | 10 | 11.3 | 1.7c | 13.0 | <2.0 | >9.3 | 86 ± 8 | ||||||
Mean | 8.8 | 2.3 | 11.1 | <2.0 | >6.8 | 85 ± 4 | ||||||||
E14.5 | GFP − | 50 | 40 | 10.9 | 3.9 | 14.8 | <2.0 | >8.9 | 75 ± 3 | |||||
GFP + | 50 | 60 | 16.7 | 2.4c | 19.1 | <2.0 | >14.7 | 71 ± 2 | ||||||
|
Mean |
|
|
14.4 |
3.0 |
17.4 |
<2.0 |
>12.4 |
73 ± 2 |
Mean values of cell-cycle parameters for the sum of GFP− and GFP+ NE/RG cells were calculated using their proportion at E11.5 and E15.5 (t24 h). GF, Growth fraction; TC, total length of the cell cycle; TS, length of the S phase; TG2 + M, length of the G2 phase plus the M phase; TG1, length of the G1 phase.
Data from Haubensak et al. (2004).
Calculated according to Nowakowski et al. (1989). TG2 + M is only given for the apically dividing NE/RG cells.
Values are underestimations (see supplemental material, available at www.jneurosci.org).