Figure 3.

Local effects of zymosan application on the DRG. A, Confocal photomontage of a zymosan-injected DRG and its contiguous dorsal root demonstrating an increase in the growth-associated protein GAP-43 (green). B, Inset of A at a higher magnification to better accentuate the increases in GAP-43 expression with zymosan injection. C, A saline-treated control DRG demonstrates a paucity of GAP-43 expression in the root. D, Control DRG immunostained for ED-1 (green), indicating an absence of invading activated immune cells. E, At 7 d after zymosan injection, a marked influx of ED-1-positive macrophages can be identified in the DRG (green). F, Photomicrograph of the same DRG showing Texas Red-conjugated zymosan particles. G, Overlay of E and F demonstrates that the zymosan particles colocalize with the invading ED-1-positive macrophages. Scale bars: A–C, 50 μm; D–G, 100 μm.