Figure 4.

Multiple strategies to attempt to promote regeneration across the DREZ. A, A C8 dorsal root crush that received no treatment demonstrates a lack of regeneration through the DREZ (thin arrow) into the spinal cord despite the ability to extend axons through the crushed root (fat arrow). Axons are labeled with dextran–Texas Red. B, Animals that received zymosan alone 7 d before injury demonstrated slight regeneration through the DREZ (thin arrow) into the dorsal gray. The DREZ is highlighted by the presence of CSPG staining (green). C, Animals that received ChABC alone demonstrated very slight regeneration of fibers (thin arrow) through the DREZ (thick arrow). Areas of CSPG digestion from the ChABC injection can be readily identified by anti-2-B-6 staining (green). Scale bar, 50 μm.