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. 2005 Jan 12;25(2):352–362. doi: 10.1523/JNEUROSCI.3887-04.2005

Figure 1.

Figure 1.

Axonal expression and FRAP analysis of synaptophysin-EGFP. a, Neuron transfected with synaptophysin-EGFP (green) and immunolabeled with antibodies directed against tau (red). Synaptophysin-EGFP is localized in the axon. Area selected for inset as indicated. Scale bar, 10 μm. b, Neuron transfected with synaptophysin-EGFP (green) and immunolabeled with an antibody directed against VAMP2 (red). Colocalization between synaptophysin-EGFP and VAMP2 demonstrates expression of synaptophysin-EGFP in synaptic vesicle precursors. Area selected for inset as indicated. Scale bar, 10 μm. c, FRAP of an axon from a synaptophysin-EGFP-transfected neuron. The bleached area of 20 μm in length (white circle) was scanned by confocal microscopy over time, and fluorescence recovery intensity was determined. Selected images before bleaching and 120, 240, 360, 480, and 600 sec after bleaching are shown. Scale bar, 10 μm. d, Relative FRAP intensity of synaptophysin-EGFP in an axon that was time-lapse scanned over 1200 sec. Individual values of each 10 sec and the curve of the equation are shown.