Skip to main content
. 2005 Sep 21;25(38):8776–8787. doi: 10.1523/JNEUROSCI.2650-05.2005

Figure 9.

Figure 9.

Kir2 and Kleak K+-channel blockade occludes the effects of ZD-7288 on EPSP summation. A, Whole-cell current-clamp records (EPSPs) from a PPN during 40 Hz, 400 μA stimulation (black line) in the control condition (red trace), after partial Kir2/Kleak blockade after Ba2+ application (50 μm, blue trace). B, EPSPs during Kir2/Kleak block (blue trace in A) and both Kir2/Kleak and HCN blockade after ZD-7288 application (50 μm each, green trace). C, Voltage traces from A and B, normalized to the amplitude of the first EPSP in the train, show that the increase in temporal summation seen under conditions in which Kir2/Kleak channels are blocked is essentially unaltered after HCN channel blockade. D, Box plot of the ratio of the last to first EPSP in the train illustrates that the temporal summation seen under conditions in which Kir2/Kleak current is blocked (blue box) is not significantly increased with subsequent HCN channel blockade (green box), suggesting Kir2/Kleak currents are necessary to maintain HCN channel effects on synaptic integration (Mann–Whitney U test; p < 0.05; n = 4).